Fig. 2

Replication kinetics in LACV-infected iPSC-derived human COs. a COs were collected at indicated timepoints for evaluation of viral RNA via qRT PCR with virus-specific primers. Viral RNA levels for each sample were calculated as the percentage difference in threshold cycle (C_T) value (ΔC_T = C_T for Gapdh gene-C_T for virus). Viral mRNA was plotted as the percentage of gene expression relative to that of the Gapdh gene. Open circles indicate mock COs, closed squares COs infected with 10³ PFU, and open squares CO infected with 10¹ PFU. Each point represents one CO. b Media supernatants were collected from individual wells containing COs at the indicated time point and assayed for PFU/mL by plaque assay. Data are representative of n = 6–12 mock, n = 6–24 LACV 10³, and n = 3 LACV 10¹ COs