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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: The spinal microglial IL-10/β-endorphin pathway accounts for cinobufagin-induced mechanical antiallodynia in bone cancer pain following activation of α7-nicotinic acetylcholine receptors

Fig. 2

Effects of cinobufagin on spinal gene (ac) and protein (d, e) expression of IL-10, β-endorphin and dynorphin A in bone cancer pain rats. The spinal lumbar enlargement (L3-L5) of female bone cancer pain rats were collected 1 h after intrathecal injection of cinobufagin. The mRNA expression of IL-10, the β-endorphin precursor proopiomelanocortin (POMC) and dynorphin precursor prodynorphin (PDYN), and protein expression of IL-10 and β-endorphin were measured by using qRT-PCR and commercial fluorescent immunoassay kits, respectively. The data are presented as means ± SEM (n = 6 per group). The asterisk denotes statistical significance (p < 0.05) compared to the saline control group, by unpaired and two-tailed Student t test

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