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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: The spinal microglial IL-10/β-endorphin pathway accounts for cinobufagin-induced mechanical antiallodynia in bone cancer pain following activation of α7-nicotinic acetylcholine receptors

Fig. 4

Effects of the IL-10 antibody (a, b) and β-endorphin antiserum (c, d) on cinobufagin-stimulated gene expression of IL-10 and β-endorphin precursor proopiomelanocortin (POMC) in primary cultures of spinal microglia. Microglia cells, originated from the spinal cords of 1-day-old neonatal rats, were first treated with the IL-10 antibody or β-endorphin antiserum for 2 h followed by cinobufagin, and were collected 2 h after the cinobufagin treatment. The mRNA expression of IL-10 and POMC was measured using qRT-PCR. The data are presented as means ± SEM (n = 3 independent repeats with duplicates). The asterisk denotes statistical significance (p < 0.001) compared to the control group, by unpaired and two-tailed Student t test

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Journal of Neuroinflammation

ISSN: 1742-2094