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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Proteomic analysis of cerebrospinal fluid extracellular vesicles reveals synaptic injury, inflammation, and stress response markers in HIV patients with cognitive impairment

Fig. 7

Effect of oxidative stress on astrocytic, stress response, and inflammation markers in U87 cells and EVs. a Cultured U87 cells were treated with 10, 25, 50, 100, 250, 500, and 1000 μM H2O2 for 4 h. Cell viability was measured by MTT assay (n = 3). Data represent mean ± SEM. b U87 cells were treated with H2O2 (50, 100, 250, and 500 μM) for 4 h and generation of intracellular ROS was measured spectrophotometrically (n = 3). Significant differences in a and b were evaluated by t test (p value < 0.05). c Fluorescence images of intracellular ROS following H2O2 treatment (50, 100, 250 μM) of U87 cells for 4 h. Scale bar = 100 μm. d Immunoblotting for astrocytic (GFAP, GLUL) and stress response (PRDX2, PARK7) markers in U87 cells and EVs (n = 3). GAPDH and CD9 were used as loading controls for U87 cells and U87 EVs, respectively. Results are representative of three independent experiments. e Densitometric quantification of normalized GFAP, GLUL, PARK7, and PRDX2 bands from U87 cells and EVs. Significant differences between at least two of the four conditions in each set are indicated using one-way ANOVA (p value < 0.05)

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