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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: TRPV1 translocated to astrocytic membrane to promote migration and inflammatory infiltration thus promotes epilepsy after hypoxic ischemia in immature brain

Fig. 2

Expression and distribution of TRPV1 in primary cultured astrocytes after OGD. a Schematic of experimental design. b TRPV1 transcripts were detected in astrocytes by RT-PCR. M: DNA marker. c The membrane protein of TRPV1 in immunoreactive bands and quantitative analysis after OGD. d Western blots and quantification for GFAP and TRPV1 of total protein in astrocytes. e Confocal images showed cellular localization patterns of TRPV1 (red) in GFAP (green) astrocytes with the nucleus stained with DAPI (blue). White arrows indicated the distribution of TRPV1 in astrocytes. White line analyzed the distance between TRPV1 and the DAPI in astrocytes and analyzed in f. The intensity of GFAP was determined in 5 fields/well and divided by the number of cells counterstained with DAPI. g Relative size of astrocyte. h Co-localization between GFAP and TRPV1 by using the PCC method. i The intensity and distribution of different colored pixels of GFAP (green) and TRPV1 (red) in astrocytes. *P < 0.05, ** P < 0.01 ***P < 0.001. Scale 10 μm. All data: mean ± SEM. Unpaired Student’s t test

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