Fig. 2
From: Nav1.6 promotes inflammation and neuronal degeneration in a mouse model of multiple sclerosis
Chronic stage EAE mice have increased RGC survival in retinas with reduced Scn8a (Nav1.6). a A population of RGCs (RBPMS-positive) in a normal (−EAE/−AAVCre) retina is shown in comparison to b a representative image of an uninjected (−AAV) EAE mouse, and c a representative image of a EAE mouse retina from a control AAVGFP-treated eye (+EAE/+AAVGFP) showing RBPMS-positive degenerating RGCs (white arrowheads) with GFP occasionally co-localizing with cell remnants. d A representative image of an EAE mouse retina from an AAVCre-treated eye (+EAE/+AAVCreGFP) showing normal appearing GFP-positive RGCs. e RGC quantification in +EAE retinas treated with AAVGFP (n = 3) or AAVCreGFP (n = 3). Lines link data points for retinas from the same animal. f Percent of expression change for Scn8a and Rbpms in AAVCre-treated (+EAE/+AAVCreGFP; n = 4) eyes relative to their contralateral control uninjected (+EAE/−AAV; n = 4) or GFP (+EAE/+AAVGFP; n = 4) eye. Scale bar = 50 μm. Data are presented as the mean ± SEM. *P ≤ 0.05, paired t test