Fig. 4

Targeting of Nav1.6 results in reduced infiltration of myeloid cells in EAE optic nerves. a Hematoxylin and eosin-stained optic nerves from control non-EAE-induced/uninjected eye (−EAE/−AAVCre) and from EAE-induced (+EAE) mice with eyes left uninjected (−AAVCre, uninj), injected with AAVGFP (+AAVGFP), or injected with AAVCreGFP (+AAVCre). Arrowheads indicate cellular aggregates. b Quantification of optic nerve nuclei from non-EAE-induced/uninjected eyes (−EAE/−AAVcre, open triangles) and from EAE-induced mice where a comparison is made between AAVCreGFP-injected (+AAVCre, black circles; n = 7) and the contralateral eye, which is either left uninjected (blue circles; n = 2) or injected with AAVGFP (green circles; n = 5). c Representative dot plot flow cytometry analysis showing the gating strategy used to identify the macrophage population expressing marker CD11b⁺ F4–80⁺ from a population of CD45⁺ cells isolated from optic nerves. d Flow cytometry analysis for CD11b⁺ F4–80⁺ macrophages. Lines link data points for retinas from the same animal. Scale bar, 500 μm. Data are presented as the mean ± SEM. *P ≤ 0.05, **P ≤ 0.01, paired t test