Fig. 6

Deletion of IL-33 results in more severe neuron degeneration following RD. a–b Scotopic ERGs of WT and IL-33^−/− at day 28 post-RD. Line graph of A-wave amplitudes (a) and B-wave amplitudes (b) at flash intensities ranging from 0.008 to 25 cd s/m² were quantified. Data were presented as mean ± SEM, n = 5–12 eyes per experimental condition; arrow, day 28 post-RD. Student’s t test was used to compare the amplitudes between WT and IL-33^-/- at the same flash intensity. *P < 0.05. c–e Photoreceptor degeneration in WT and IL-33^−/− retina at day 28 post-RD was investigated using immunohistochemistry of cone-arrestin. c The number of nuclear layer of the ONL in normal retina, non-detached area and detached area of RD retina. d The number of rod cells, cone-arrestin⁺ cells in normal retina, non-detached area and detached area of RD retina. e Representative images of WT and IL-33^−/− retinal sections stained for cone-arrestin (red) and DAPI (blue). f–g Synaptic integrity in WT and IL-33^−/− retina at day 28 post-RD was investigated using immunohistochemistry of synaptophysin. f The area of synaptophysin in OPL and IPL in normal retina and detached and non-detached regions of RD retina. g Representative images of WT and IL-33^−/− retinal sections stained for synaptophysin (green) and DAPI (blue). GCL ganglion cell layer, INL inner nuclear layer, ONL outer nuclear layer, OPL outer plexiform layer, IPL inner plexiform layer, CON control retina, ND non-detached region of retina having RD, DE detached region of retina having RD. Data presented as mean ± SEM. n = 5 mice, 3–6 sections per retina. Student’s t test was used to compare the means between WT and IL-33^-/- at the same condition. *P < 0.05, **P < 0.01. Scale bar, 50 μm