Fig. 7

Primary Müller cells from WT and IL-33^−/− mice present similar inflammatory response. a mRNA levels of CCL2, IL-6, IL-18, VEGF, TGFβ, GFAP, GS in WT and IL-33^−/− PMC cultured in standard incubators. Expression levels of CCL2 and IL-6 was lower in IL-33^−/− PMC, whereas other genes were at similar levels. b mRNA level of PMCs cultured in 1% hypoxia for 24 h. Expression level was compared to PMC from the same strain. Fold changes above the dotted line suggested increased response in hypoxia (VEGF); fold changes below the dotted line suggested decreased response in hypoxia (CCL2, IL-6 and IL-18). c, d Cytokine concentration in culture supernatants were measured by CBA. Hypoxia reduced the secretion of CCL2 and IL-6 in both WT and IL-33^−/− PMCs. Data presented as mean ± SEM, n = 3. a, b Student’s t test. c, d Two-way ANOVA followed by Tukey’s multiple comparisons. n = 6, *P < 0.05, **P < 0.01, ***P < 0.001. CBA cytometric beads array