Fig. 8

Bone marrow-derived macrophages from IL-33^−/− mice are more pro-inflammatory after classic stimulation (LPS/IFNγ). a, b mRNA expression of inflammatory genes including iNOS, TNFα, IL1-β, CCL2 and IL-6 before (a: M0) or after (b: M1) LPS + INFγ treated BM-DMs cultured from WT and IL-33^−/− mice. Y-axis showing the fold changes of mRNA expression level compared to non-stimulated BM-DM. Dot line levels fold change at 1. RNA levels were upregulated after stimulation for each cell type. The increased levels of iNOS, TNFα, IL-18 and CCL2 of IL-33^−/− BMDMs were significantly higher than those of WT BM-DMs. c Secretion of TNFα was higher in IL-33^−/− BM-DM than in WT. d The concentration of NO released into the culture medium from IL-33^−/− BM-DM was higher than those in WT BMDM. NO nitric oxide. Data presented as mean ± SEM, n = 3, Student’s t test, *P < 0.05, **P < 0.01, ***P < 0.001