Table 1 Antibodies used for immunohistochemistry and western blot
From: IL-33 deficiency causes persistent inflammation and severe neurodegeneration in retinal detachment
Antibody | Dilution | Company | Catalogue number |
|---|---|---|---|
CD68 | 1:300 | BioRad | MCA1957 |
Cone-arrestin | 1:1000 | Millipore | AB15282 |
GFAP | 1:200 | DAKO | Z0334 |
GS | 1:2000 | Sigma | G2781 |
IL-33 | 1:50 | R & D | AF3626 |
Donkey anti-goat IgG Alexa Fluor 488 | 1:300 | Jackson Immunoresearch | 705–545-147 |
Donkey anti-rabbit IgG Alexa Fluor 488 | 1:300 | Jackson Immunoresearch | 711–585-152 |
Goat anti-rat IgG Alexa Fluor 594 | 1:300 | Invitrogen | A-11007 |