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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Distinct migratory pattern of naive and effector T cells through the blood–CSF barrier following Echovirus 30 infection

Fig. 3

E-30 infection of HIBCPP cells enhances migration of effector T cells. Migration of Th1 effector and CVB4-specific T cells across HIBCPP cells was evaluated via immunofluorescence imaging in the following conditions: UI, UI + CXCL12, E-30 and E-30 + CXCL12. T cells were stained in cell tracker green. Immunofluorescence imaging (10 FOV per conditions) of Th1 (a) and CVB4-specific T cells (c) present in the bottom well was taken after 4 h of migration across HIBCPP cells for every condition. b, d Quantification of Th1 (b) and CVB4-specific T cells (d) present in the bottom well after 4 h of migration for all the conditions. The data are shown as mean ± SD of 6 independent experiments each performed in triplicate. Statistical significance was calculated using a Tukey–Kramer test. p values are displayed as follows: *p < 0.05 and *** p < 0.0001. *p < 0.05 was reached comparing UI to E-30; p < 0.0001 (***) was reached comparing UI to UI + CXCL12, and UI to E-30 + CXCL12; p < 0.0001 (###) was reached comparing E-30 against E-30 + CXCL12. The white scale bar represents 100 μm

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