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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Distinct migratory pattern of naive and effector T cells through the blood–CSF barrier following Echovirus 30 infection

Fig. 4

Th1 and CVB4-specific T cells are retained at the basolateral side of HIBCPP cells after of migration. T cell association with HIBCPP cells after 4 h was analyzed by immunofluorescence microscopy. The following conditions were analyzed: UI, UI + CXCL12, E-30, E-30 + CXCL12. a shows representative images for CD3+, b for CD4+, c for CD8+, d for Th1, and e for CVB4-specific T cells. Per condition, 10 FOV were taken with X63/1.4 objective lens. T cells were stained in cell tracker green Alexa 488 and nucleus in blue (DAPI). f Quantification of the T cells associated to the basolateral side of HIBCPP cells after 4 h for the following conditions UI, UI + CXCL12, E-30, E-30 + CXCL12 and the following T cell subsets: CD3+, CD4+, CD8+, Th1, and CVB4-specific T cells are shown. Data are shown as mean ± SD of 6 independent experiments each performed in triplicate. Statistical significance was calculated using a Tukey–Kramer test. p values are displayed as follows: p < 0.0001 (****). p < 0.0001 (****) was reached comparing all the values for each condition from effector T cells and CVB4-specific T cells with the values for each condition from naïve T cells. The white scale bar represents 100 μm

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