Fig. 8

Shape of T cells differs after the migration process depending on the stimulation condition. The shape of the T cells after the migration process from all the T cells subtypes CD3⁺, CD4⁺, CD8⁺, Th1 effector, and CVB4-specific T cells was determined in the following conditions: UI, UI + CXCL12, E-30, E-30 + CXCL12. T cells were labeled in green. The shape of migrated naïve T cells without CXCL12 could not be determined due to low migration rates in uninfected conditions. a Immunofluorescence microscopy pictures show “round” (first line) and “polarized” shape of T cells observed 4 h after migration across HIBCPP cells. Quantification of CD3⁺ (b), CD4⁺ (c), CD8⁺ (d), Th1 (e), or CVB4-specific T cells (f) with a “round” versus “polarized” shape after the migration process. The data are shown as mean ± SD of 6 independent experiments each performed in triplicate. Statistical significance was calculated using a Tukey–Kramer test. p values are displayed as follows: p < 0.05 (*), p < 0.0001 (***), p < 0.05 (#), p < 0.0001 (###). p < 0.0001 (***) were reached comparing UI + CXCL12 to E-30 for all the T cell subsets. p < 0.0001 (***) was also reached comparing UI + CXCL12 to E-30 + CXCL12 for CD8⁺, Th1 and CVB4-specific T cells. p < 0.05 (*) were reached comparing UI + CXCL12 to E-30 + CXCL12 for CD3⁺ and CD4⁺. p < 0.05 (#) was reached comparing E-30 against E-30 + CXCL12 for CD8⁺. p < 0.0001 (###) was reached comparing UI and UI + CXCL12 against E-30 and against E-30 + CXCL12, respectively, for Th1 and CVB4-specific T cells