Fig. 3From: Midbrain microglia mediate a specific immunosuppressive response under inflammatory conditionsAnalysis of inflammatory cell surface markers on microglial cells after LPS exposure. Microglial cells were purified from the cortex (Ctx), hippocampus (Hipp), midbrain (Mdb), and striatum (Str) from control and LPS-treated mice. The expression of inflammatory cell surface markers was assayed in the CD45lowCD11b+ fraction of cells by flow cytometry. Data obtained were analyzed by two-way ANOVA followed by pairwise comparisons with Šidák adjustment to determine the effect of LPS in each region. Significant p values for interaction (Pint) were validated with a permutation test (Per test). The region effect in control animals was analyzed by one-way ANOVA followed by a contrast analysis with Šidák adjustment. a Percentage of CD45lowCD11b+ cells expressing TLR4, Pint < 0.001, Per test 95% CI 0–0.004. b MFI of CD40. c MFI of MHC-I. d Percentage of MHC-II cells, Pint < 0.001, Per test 95% CI 0–0.004. e MFI of CD86, Pint < 0.001, Per test 95% CI 0–0.003. f MFI of CD86. The data are from eight to nine animals per group and are represented as the mean ± 95% CI. Significant effect of LPS in each region: **p < 0.01, ***p < 0.001. Significant differences between regions in control animals: ##p < 0.01, ###p < 0.001Back to article page