From: Midbrain microglia mediate a specific immunosuppressive response under inflammatory conditions
Antigen | Fluorophore | Clone | Dilution | Trademark |
---|---|---|---|---|
CD3ε | PerCP-Vio770 | 145-2C11 | 1:50 | Miltenyi Biotec |
CD4 | FITC | GK1.5 | 1:1000 | BioLegend |
CD8a | PE-Cy7 | 53.6-7 | 1:1000 | BioLegend |
CD11b | VioBlue | M1/70.15.11.5 | 1:100 | Miltenyi Biotec |
CD25 | APC | PC61 | 1:200 | Biolegend |
CD28 | APC | 37.51 | 1:50 | Biolegend |
CD40 | PE | 3/23 | 1:200 | Biolegend |
CD45 | BV510 | 30F11 | 1:1000 | Biolegend |
CD80 | APC | 16-10A1 | 1:100 | Biolegend |
CD86 | APC | GL-1 | 1:100 | Biolegend |
MHC class I | FITC | 28-8-6 | 1:200 | Biolegend |
MHC class II | PE | AF6-120.1 | 1:1500 | Biolegend |
TLR4 | PE-Cy7 | SA15-21 | 1:500 | Biolegend |
PD-L1 | PE | MIH5 | 1:100 | BD Bioscience |
Foxp3 | PE-Cy5.5 | FJK-16s | 1:50 | Invitrogen |
CTLA-4 | PE-Cy7 | UC10-4B9 | 1:100 | BioLegend |