Skip to main content

Table 1 Primary antibodies used for flow cytometry analysis

From: Midbrain microglia mediate a specific immunosuppressive response under inflammatory conditions

Antigen

Fluorophore

Clone

Dilution

Trademark

CD3ε

PerCP-Vio770

145-2C11

1:50

Miltenyi Biotec

CD4

FITC

GK1.5

1:1000

BioLegend

CD8a

PE-Cy7

53.6-7

1:1000

BioLegend

CD11b

VioBlue

M1/70.15.11.5

1:100

Miltenyi Biotec

CD25

APC

PC61

1:200

Biolegend

CD28

APC

37.51

1:50

Biolegend

CD40

PE

3/23

1:200

Biolegend

CD45

BV510

30F11

1:1000

Biolegend

CD80

APC

16-10A1

1:100

Biolegend

CD86

APC

GL-1

1:100

Biolegend

MHC class I

FITC

28-8-6

1:200

Biolegend

MHC class II

PE

AF6-120.1

1:1500

Biolegend

TLR4

PE-Cy7

SA15-21

1:500

Biolegend

PD-L1

PE

MIH5

1:100

BD Bioscience

Foxp3

PE-Cy5.5

FJK-16s

1:50

Invitrogen

CTLA-4

PE-Cy7

UC10-4B9

1:100

BioLegend