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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Innate immunity activation in the early brain injury period following subarachnoid hemorrhage

Fig. 3

SAH induction was locally associated with activation of microglia and pro-inflammatory monocytes, and neutrophil recruitment. Flow cytometry analyses of brain infiltrating leukocytes. a Representative dot plot for quantification of neutrophil percentages (CD11b+Ly6G+) at 12 h (nSHAM = nSAH = 8), D1 (nSHAM = 16, nSAH = 18), D2 (nSHAM = 16, nSAH = 15) and D7 (nSHAM = nSAH = 8). We observed a significant increase in neutrophils following SAH compared with SHAM at 12 h (*p = 0.0080), D1 (*p = 0,030) and D2 (*p = 0.017). Neutrophils were almost undetectable at D7. Values from the normal group showed no significant differences between normal and SHAM groups. b Representative dot plot and quantification of positive cell percentages of microglia (CD45lowCD11blow), activated microglia and macrophages (CD45highCD11bhigh) and leukocytes (CD45+CD11b-) at 12 h, D1, D2, and D7. We observed a significant decrease in microglia at 12 h (*p = 0.0286) and a significant increase in activated microglia at D1 (*p = 0.031) and D2 (*p = 0.0085) following SAH compared with SHAM. No significant differences were observed for microglia and leukocytes over time using this strategy. No significant differences were observed between normal and SHAM groups. c Representative dot plot and quantification of positive cell percentages for pro-inflammatory (Ly6G-CD11b+Ly6Chigh) and anti-inflammatory (Ly6G-CD11b+Ly6Clow) monocytes at 12 h, D1, D2, and D7. We observed a significant decrease in non-classical monocytes at 12 h (*p = 0.0225) followed by a significant increase in pro-inflammatory monocytes at D1 (*p = 0.049) and D2 (*p = 0.020) following SAH compared with SHAM. Monocytes, either classical or non-classical, were almost undetectable at D7. There was no significant difference between normal and SHAM groups

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