Skip to main content
Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Innate immunity activation in the early brain injury period following subarachnoid hemorrhage

Fig. 4

SAH induces a local activation of microglia and astrocytes, and caspase-3-positive apoptotic neurons. Immunofluorescence staining was performed at D7 on brain slides (nSHAM = nSAH = 11). a Representative pictures of activated microglia (Iba-1, pink) and astrocytes (GFAP, yellow) are shown for the different groups (Normal, SHAM, and SAH). Quantification of pixels showed that SAH significantly induced microglia (*p = 0.009) and astrocyte (*p = 0.021) activation compared with control (SHAM). b Representative pictures of neurons (NeuN, yellow) and activated caspase-3 (cleaved caspase-3, red) are shown for the different groups (Normal, SHAM, and SAH). Merged images showing colocalization define apoptotic neurons. Quantification showed that SAH induced a significant increase in neuron death compared with control (*p = 0.010). DAPI was used as a counterstain for nuclei (bar = 50 μm).

Back to article page