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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Progranulin deficiency exacerbates spinal cord injury by promoting neuroinflammation and cell apoptosis in mice

Fig. 5

PGRN deficiency aggravates neuroinflammation and apoptosis after SCI. a At day 7 after SCI, total protein was extracted from injured spinal cord of both WT and PGRN-deficient mice, and iNOS, p-p65, p65, and GAPDH were detected by Western blotting. b Quantification of iNOS/GAPDH in (a). n = 3 per group. c Quantification of p-p65/p65 in (a). n = 3 per group. d Immunofluorescence of iNOS and CD68 in both WT and PGRN-deficient mice at day 7 after SCI. Images were taken at the anterior horn of gray matter. Arrows indicate co-localization of iNOS and CD68. e At day 7 after SCI, total protein was extracted from injured spinal cord of both WT and PGRN-deficient mice, and Bax, Bcl-2, and GAPDH were detected by Western blotting. f Quantification of Bax/GAPDH in (e). n = 3 per group. g Quantification of Bcl-2/GAPDH in (e). n = 3 per group. h At day 7 after SCI, apoptotic neurons in the ventral horn were detected by TUNEL assays. i Quantification of apoptotic neuron numbers in (h). n = 3 for each group. *p < 0.05, ** p < 0.01 WT-SCI vs Grn−∕−-SCI groups. ns, not significant

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