Fig. 2

Intrathecal delivery of BMSCs but not fibroblasts transiently attenuated mechanical allodynia. a CCD induced significant mechanical allodynia ipsilaterally (measured by foot lift response frequency to stimulation with 12 g von Frey filament), and sham surgery produced transient hypersensibility compared to the baseline value. BMSCs (i.t.) induced a significant (**p < 0.01) reduction of mechanical allodynia at 6 days postoperative compared to the control (fibroblasts and PBS), but lack of long-lasting effects (< 2 days). Compared to the PBS control, pro-inflammatory fibroblasts induced transient hypersensitivity ipsilaterally in sham animals (*p < 0.05). b PBS and BMSC injection have no influence on the nociceptive behavioral of the contralateral hind paws, while fibroblast induces transient hypersensitivity (**p < 0.01). c Licking times after mechanical stimulation increased at days 4 post CCD surgery (^##p < 0.01). d Sensorimotor coordination using the rotarod test shows no difference was observed between pre-injection and one day after second BMSC injection (day 4 and day 6). Each time point had a sample size of 9, **p < 0.01 compared with CCD + PBS control; *p < 0.05 compared with sham + PBS control. ^##p < 0.01 compared with sham control