Fig. 4

a RT-PCR analysis for β1-AR, β2-AR, and β3-AR in the CCx, HC, TM, HT, amygdala (Amy), SN, and peripheral organs, such as the adrenal gland (AG), spleen (SPL), and intestine (INT). β-Actin was used as an internal control. b ISH and IHC for β1-AR, β2-AR, and β3-AR in the HT. Scale bar: 10 μm. c, d Double immunofluorescence for OX-42 and β1-AR, β2-AR, and β3-AR in the HT (c) and GFAP and β1-AR, β2-AR, and β3-AR in the HT (d), respectively. Scale bar: 20 μm