Fig. 3

Altered microglial morphology and distribution in VPS35^CX3CR1 cortex after ischemic stroke. The ischemic stroke experiment was carried out as described in the legend of Fig. 1a. Three days after the stroke, mouse brain sections were subjected to co-immunostaining analyses using indicated antibodies. a, b Representative images. Iba-1, a marker for microglia/macrophages. c, d Quantification analyses. Cell densities for GFP⁺;Iba1⁺ and GFP⁻(negative);Iba1⁺ microglia/macrophages were shown (mean ± SEM; n = 5/group; *P < 0.05). e GFP fluorescence intensity per Iba1⁺ cell was shown (mean ± SEM; n = 5/group; *P < 0.05)