Fig. 7
Expression of factors associated with inflammatory and anti-inflammatory microglia in VPS35CX3CR1 cortex after ischemic stroke. RT-PCR analysis was performed using total RNAs extracted from ischemic brains (of control and Vps35 mutant mice) at 6 h and 1, 3, and 7 days after stroke and primers for genes associated with anti-inflammatory microglia (e.g., Arg1, YM1/2, and CD206) or pro-inflammatory microglia (e.g., CD86, CD32, and iNOS). a Increased expression of factors associated with anti-inflammatory microglia, but decreased expression of factors associated with inflammatory microglia, in VPS35 mutant cortex 3 days after stroke. Data are expressed as fold change (mutant/controls). n = 4 per group. *P < 0.05. b Reduced expression of factors associated with inflammatory microglia in VPS35 mutant cortex at indicated time point post-stroke. c Increased expression of factors associated with anti-inflammatory microglia in VPS35 mutant cortex at indicated time point post-stroke. Data in b and c are expressed as fold change vs sham-operated controls. n = 4 per group. *P < 0.05