Fig. 1

TLR4 knockout increases survival and reduces behavioral scores in ICH-induced mice. a Immunofluorescence staining of brain tissue for IBA-1 (488 nm), NeuN (647 nm), and GFAP (555 nm) in hemorrhagic site. b Changes in TLR4 expression at the first, third, and seventh day after inducing ICH. c Detection of TLR4 expression in BV2 cells treated with collagenase VII for 48 hours. d Quantification of TLR4 expression in panel c. e Transcriptional level of TLR4. f Interaction of TLR4 with collagenase VII as detected by immunoprecipitation ; positive control CD14. g Genetic identification of wild-type and knockout mice using mutant TLR4 primers and wild-type TLR4 primers. h Kaplan-Meier survival analysis of 10 TLR4+/+ mice and 10 TLR4−/− mice. i Behavioral Longa scores of ICH-induced mice on postoperative days 1, 3, 5, and 7. The difference was statistically significant. *P < 0.05 represents a statistically significant difference between the two groups