Fig. 2

Differential expression of apoptosis-related proteins in TLR4 knockout mice and wild-type mice after ICH induction. a The expression of Caspase 3, 8, 9, Bcl-1 and Bax in the brain tissue around the bleeding site of TLR4 knockout mice and wild-type mice on days 1, 3, and 7 after ICH induction. b Quantification of caspase 9. c Quantification of caspase 8. d Quantification of caspase 3. e Quantification of Bax. f Quantification of Bcl-2. Western total protein was 30 μg. GAPDH is used as a housekeeping protein to prove the equal loading in each lane of the electrophoresis and normalize densitometric values of the other protein analyzed. Each experiment was repeated three times. *P < 0.05 represents a statistically significant difference between the two groups