Fig. 3

Deletion of sEH recovers nesting building ability, spatial learning, and memory in APP/PS1 Tg mice. a PCR analysis for genotyping of representative animals depicting WT, sEH^−∕−, APP/PS1 Tg, and APP/PS1 Tg/sEH^−∕−. PCR bands of 608 bp for PS1, 377 bp for APP, 338 bp for sEH^+/+, and 295 bp for sEH^−∕− were amplified for genotypes. b, c Representative examples and the score for nest building for 6-month-old WT, sEH^−∕−, APP/PS1 Tg, and APP/PS1 Tg/sEH^−∕− mice. d–g Schematic diagram of Y-maze experimental design and ratio of first entry, number of entries, and distance moved in the novel arm. h Morris water maze (MWM) test of learning patterns verified on days 1 to 4. i–j At day 5 after training, the number of times of crossing the hind platform and retention times in the target quadrants. k The latency of arrival to the visible platform. Data are mean ± SEM from 8 mice in each group. c, e–g, i, and j *P < 0.05 vs. WT mice. ^#P < 0.05 vs. APP/PS1 Tg mice (i). *P < 0.05 vs. day 1. ^#P < 0.05 vs. WT mice