Fig. 2

Validation of α-syn pathology in the CNS and peripheral gut tissue of C57BL/6 J mice. Stereotaxic injection of 5 μg human PFF α-syn or monomer α-syn into striatum (AP+0.3, ML+2.3, DV−3.5) of C57BL/6 mice was performed. Mice were sacrificed at 5 months p.i. (n = 5–6 animals/group). a Representative immunohistochemical images in the primary motor cortex, SNpc, striatum, and hippocampus of C57BL/6J mice. Scale bars represent 10 μm (40 × high magnification images) and 100 μm (5 × low magnification images). Graphs represent optical density (O.D.) of positive p-α-syn in the ipsilateral primary motor cortex, SNpc, striatum, and hippocampus (n = 4–6 per group). b Representative immunohistochemical images of α-syn in the striatum with or without proteinase-K treatment (10 μg/mL). Scale bars represent 10 μm. c Representative immunohistological images of p-α-syn and MAP-2 (neuronal marker) positive staining in the small intestines. Nuclear staining with DAPI shown in blue. Scale bar represents 20 μm. Asterisks denote significant differences with *p < 0.05, comparing PFF α-syn and monomer α-syn treated animals, according to Student’s t test