Fig. 4

Peripheral immune cells infiltrate in the CNS parenchyma in the PFF α-syn mice. Mononuclear cells from the CNS were isolated as described in the “Methods” section from PFF α-syn or monomer α-syn mice at 5 months p.i. (n = 6 per group). a Gating strategy for flow cytometry data analysis. b Plots show relative percentages of CD19+ B cells, T cells (CD4+ and CD8+ T cells), CD11b+CD45^low (microglia), CD11b+CD45^high (macrophage or activated microglia), Ly6+ neutrophils, and NK1.1+ NK cells (blue: monomer α-syn; red: PFF α-syn). Data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, n.s.; not significant, Student’s t test