Fig. 5

PFF α-syn–injected mice display increased total number of immune cells within the spleen. Mononuclear immune cells from the spleen were isolated from PFF α-syn or monomer α-syn mice at 5 months p.i. (n = 6 per group). a The gating strategy for lymph node, spleen, and blood flow cytometry data analyses. b Plots show the numbers of total CD45+ leukocytes, CD19+ B cells, CD4+ T cells, CD8+ T cells, CD11b+ macrophage, Ly6+ neutrophils, and NK1.1+ NK cells. c Plots show the percentages of CD19+ B cells, T cells (CD4+ and CD8+ T cells), CD11b+ macrophage, Ly6+ neutrophils, and NK1.1+ NK cells under the CD45+ gated leukocytes (blue: monomer α-syn; red: PFF α-syn). Data represent mean ± SEM. *p < 0.05 or **p < 0.01, n.s. not significant, Student’s t test