Fig. 4

Transcription and translation of JMJD3 were induced by DHEA after Hb exposure. a, b Co-localization of Iba-1 and JMJD3 demonstrated an induction of JMJD3 expression after SAH/Hb exposure that was even higher in the presence of DHEA. Magnified views are marked with dashed line boxes. Green, Iba-1; red, JMJD3; blue, DAPI; bar = 70 μm. c The relative single cell fluorescence intensity (JMJD3 in Iba-1⁺/mean of JMJD3 in Iba-1^-) was calculated and presented in a violin plot (n = 20 in sham, n = 79 in SAH, n = 43 in SAH + DHEA from 6 fields, non-specific one-way ANOVA, ****P < 0.0001). d Fluorescence intensity of JMJD3 after Hb exposure (n = 6 fields, one-way ANOVA). e, f qPCR and WB showed an induction of Jmjd3 mRNA and protein expression in microglia treated with DHEA (n = 4 in qPCR and n = 3 in WB, one-way ANOVA), ns, not significant, *P < 0.05, **P < 0.01, ***P < 0.001. Eth., ethanol