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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: DHEA Attenuates Microglial Activation via Induction of JMJD3 in Experimental Subarachnoid Haemorrhage

Fig. 6

JMJD3 mediated the anti-inflammatory effect of DHEA. The mRNA expression levels of a IL-1β, IL-6, TNF-α, and CCL2 and b two homeostasis genes were detected by qPCR (n = 5, non-parametric test for P2RY12 and one-way ANOVA for the other). The gene expression levels in the vehicle group treated with dimethyl sulfoxide (DMSO) or ethanol were set as 1. c The CM collected from microglia was added to the primary neurons for another 24 h, and neuron viability was assessed by CCK8 (n = 5, one-way ANOVA). d Representative fields of primary neurons cultured with CM from microglia exposed to different treatments for 24 h. e Neuronal morphology was investigated with Sholl analysis (n = 5, two-way ANOVA with Dunnett’s multiple comparisons test, the footnote* indicates the statistically significant difference between Hb and Hb + DHEA; the difference between vehicle and Hb is not shown; no significant difference between Hb + DHEA and Hb + DHEA + GSK-J4). Bar = 50 μm. Eth., ethanol. *P < 0.05, **P < 0.01

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