Fig. 5
From: CXCL12 is involved in α-synuclein-triggered neuroinflammation of Parkinson’s disease
CXCL12/CXCR4 mediated the migration of microglia through FAK/Src/Rac-1 signaling. a The expression levels of GTP-Rac1 and total Rac1 were detected by western blotting after 24 h of stimulation by CXCL12 with or without AMD3100. b BV-2 cells were pretreated with NSC23766 for 2 h, and the migration of BV-2 cells towards CXCL12 was measured by the Transwell assay. c Western blot analysis was used to assess the expression levels of phospho-FAK (pY397), FAK, phospho-Src (pY416), and Src after 24 h of stimulation with CXCL12. d Migration of primary microglia towards CXCL12 with or without PP2 or PF573228 was measured by the Transwell assay. e Expression of GTP-Rac1 and total Rac1 were detected by western blotting after 24 h of stimulation by CXCL12 with or without PP2 or PF573228. The data are shown as the mean ± SEM from three independent experiments. ****p < 0.0001. ***p < 0.001. **p < 0.01. *p < 0.05. BSA served as a control