Fig. 2

Knockdown of MCT1 inhibited LPS-stimulated classical microglial polarization and glycolysis rate in BV2 cells. a The interference efficiency of Lenti-siMCT1, Lenti-siMCT2, and Lenti-siMCT4, respectively, in BV2 cells (n = 6 per group and errors represent S.E.M; t test). b Quantification of the mRNA level of iNOS after treated with indicated lentivirus with or without LPS stimulation (n = 6–7 per group). c Quantification of the mRNA levels of IL-1β, IL-6, and STAT1, respectively, in each group (n = 5 per group). d Quantification of lactate levels in the cultured media in each group (n = 4 per group). e Quantification of the mRNA level of PFKFB3 in each group (n = 5–6 per group). f, g Representative immunoblots and relative quantification of iNOS, PFKFB3, and MCT1 in each group (n = 6 per group). h, i The real-time extracellular acidification rate was measured by the sequential addition of glucose, oligomycin, and 2-DG in each group (n = 5 per group). *p and ^#p < 0.05, **p and ^##p < 0.01, two-way ANOVA followed by post hoc. Lenti-siCtr, Lenti-siMCT1, Lenti-siMCT2, and Lenti-siMCT4 are abbreviated as siCtr, siMCT1, siMCT2 and siMCT4, respectively