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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Monocarboxylate transporter 1 promotes classical microglial activation and pro-inflammatory effect via 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 3

Fig. 3

Overexpression of PFKFB3 rescued MCT1 silence-induced suppression of classical microglial polarization in BV2 cells. a Representative immunoblots and relative quantification of Hif-1α in each group (n = 4 per group and errors represent S.E.M). b The overexpression efficiency of Lenti-MCT1 and quantification of the mRNA level of PFKFB3 in each group (n = 4 per group). KC7F2, an inhibitor of Hif-1α, 30 mM. c, Quantification of the mRNA level of iNOS, IL-1β, and IL-6 after treatment with Lenti-siMCT1 and Lenti-PFKFB3 with or without LPS (n = 6–9 per group). d, e Representative immunoblots and relative quantification of iNOS and STAT1 protein, respectively, 24 h after LPS stimulation in each group (n = 6 per group). f Representative immunoblots and relative quantification of STAT1 and p-STAT1 after stimulation with LPS for 3 h in each group (n = 5–6 per group). *p, #p, and &p < 0.05, **p, ##p, and &&p < 0.01, NS represents no significant difference; two-way ANOVA followed by post hoc

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