Table 1 Sequence of primers used for quantitative real-time PCR
Symbol | GenBank | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|---|
iNOS | NM_001313921 | GAGCGAGTTGTGGATTGTC | CTGCCTATCCGTCTCGTC |
Arg1 | NM_007482 | ATCTGCCAAAGACATCGT | CATCACCTTGCCAATCCC |
MCT1 | NM_009196 | GAGGTCCTATCAGCAGTATCTT | CCAGTGGTCGCTTCTTGT |
MCT2 | NM_011391 | CACTGGCTCCTTTCAATC | CTGGCTTTCTTCAGAGTTG |
MCT4 | NM_001038653 | ACTTCAACAAGCGTCGCCCTAT | TCAGTCCCTCCGCCTACCTG |
PFKFB3 | NM_001177752 | AGCCTCTTGACCCTGATA | TTCTTGCCTCTGCTGGAC |
IL-1β | NM_008361 | ATTGTGGCTGTGGAGAAG | ATCTCGGAGCCTGTAGTG |
IL-6 | NM_001314054 | TGCCTTCTTGGGACTGAT | TTGCCATTGCACAACTCTTT |
TNF-α | NM_001278601 | GGCGGTGCCTATGTCTCA | CCTCCACTTGGTGGTTTGT |
Hif-1α | NM_001313919 | CATCATCTCTCTGGATTTTG | GAAGAGGGAAACATTACATC |
β-actin | NM_007393 | CGTTGACATCCGTAAAGACCTC | CCACCGATCCACACAGAGTAC |