Table 1 Primer sequences used for PCR
Gene | Forward primer (5′→3′) | Reverse primer (5′→3′) | Size |
|---|---|---|---|
TNF-α | CCTATGTCTCAGCCTCTTCT | CCTGGTATGAGATAGCAAAT | 354 bp |
iNOS | CAAGAGTTTGACCAGAGGACC | TGGAACCACTCGTACTTGGGA | 450 bp |
IL-1β | GGCAACTGTTCCTGAACTCAACTG | CCATTGAGGTGGAGAGCTTTCAGC | 447 bp |
IL-6 | CACGATTTCCCAGAGAACATGTG | ACAACCACGGCCTTCCCTACTT | 128 bp |
IL-10 | GCCAGTACAGCCGGGAAGACAATA | GCCTTGTAGACACCTTGGTCTT | 409 bp |
MMP-3 | ATTCAGTCCCTCTATGGA | CTCCAGTATTTGTCCTCTAC | 375 bp |
MMP-8 | CCAAGGAGTGTCCAAGCCAT | CCTGCAGGAAAACTGCATCG | 180 bp |
p47phox | CGATGGATTGTCCTTTGTGC | ATCACCGGCTATTTCCCATC | 256 bp |
p67phox | CCCTTGGTGGAAGTCCAAAT | ATCCTGGATTCCCATCTCCA | 242 bp |
gp91phox | ACTGCGGAGAGTTTGGAAGA | GGTGATGACCACCTTTTGCT | 201 bp |
p22phox | AAAGAGGAAAAAGGGGTCCA | TAGGCTCAATGGGAGTCCAC | 239 bp |
TLR2 | GAGCGAGCTGGGTAAAGTAG | AGCCGAGGCAAGAACAAAGA | 528 bp |
TLR4 | GAGATGAATACCTCCTTAGTGTTGG | ATTCAAAGATACACCAACGGCTCTGA | 414 bp |
GAPDH | TTCACCACCATGGAGAAGGC | GGCATGGACTGTGGTCATGA | 245 bp |