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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Dopamine receptor D3 signalling in astrocytes promotes neuroinflammation

Fig. 1

DRD3 is selectively expressed in astrocytes but not in microglial cells. Astrocyte (top panels) and microglial (middle panels) cultures were obtained from wild-type mice as described in materials and methods. PC12 cell line was used as a positive control for DRD3 expression (bottom panels). Cells were immunostained with anti-DRD3 antibody (left panels) or an isotype-matched control (right panels) primary antibody followed by an Alexa488-coupled secondary antibody and the immunofluorescence (green) associated was analysed by fluorescence microscopy. Nuclei were stained with DAPI (blue). Representative photomicrographs are shown. A higher magnification section is inserted in the bottom left corner of each image. Bar, 10 μm

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