Fig. 8

DRD3 deficiency results in a selective and strong exacerbation in the production of fizz1 in mixed glial culture in response to IL-4. Mixed glial cultures were generated from the midbrain/striatum structures obtained from WT (white) or DRD3KO (black) mice, which were left untreated (control) or treated with LPS (1 μg/ml) or IL-4 (25 ng/ml). Twenty-four hours later, the RNA was extracted and analysed by quantitative RT-PCR. Inflammatory (a), anti-inflammatory mediators (b) and neurotrophic factors (c) were evaluated. Gapdh transcript was used as a house keeping for normalization. Data from three (nrf2, bdnf, igf1, nt3), five (ym1), six (il1b, fizz1, gdnf) and seven (inos, arg1) independent experiments is shown. Values are the mean ± SEM. *p < 0.05 by two-way ANOVA followed by Sidak’s post-hoc test