Fig. 4

Immunochemical staining of amyloid plaques. a, c Representative images of of amyloid plaques with a plaque-specific antibody (4G8) in the frontal cortex and the hippocampus. Zoomed-in image demonstrating typical morphology of amyloid deposit were presented in the bottom right corner of each image. Scale bar: 100 μm. b, d The number of amyloid plaques was analyzed using ImageJ. (unpaired Student’s t tests, compared to sedentary mice, *p < 0.05, **p < 0.01. n = 5, data present as mean ± SEM). SED sedentary, RE resistance exercise