Fig. 2
From: IL-1β induces rod degeneration through the disruption of retinal glutamate homeostasis
IL-1β increases extracellular glutamate concentration that can induce rod photoreceptor degeneration similar to IL-1β. a Glutamate concentration (μM) in the 18 h supernatant of mouse retinal explants cultured with or without IL-1β (50 ng/ml) (n = 8/group, Mann–Whitney test, *p = 0.0152). b Orthogonal projection of confocal Z stack images of the outer nuclear layer of TUNEL (red)- and Hoechst nuclear dye (blue)-stained mouse retinal explants after 18 h of culture with or without glutamate (1 mM), and quantification of TUNEL+ nuclei (n = 4/group, Mann–Whitney test, *p = 0.0286). c Oblique and perpendicular (Inset)—3D reconstructions of confocal Z stack images of the PR segments of mouse retinal explants after 18 h of culture with or without glutamate (1 mM), after peanut agglutinin (PNA, green) staining, and quantification of PNA+ outer segment volume (μm3) (n = 5/group, Mann–Whitney test)