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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: IL-1β induces rod degeneration through the disruption of retinal glutamate homeostasis

Fig. 3

IL-1β disrupts glutamate homeostasis in Müller glial cells. a RT-qPCR of Eaat1 mRNA normalized with the mean of 3 HKG mRNAs in (left) retinal cell fractions (MCs: CD11b+ cells; Müller cells: CD11bCD31CD29+ cells; neurons: CD11bCD31CD29 and PRs) isolated from total retina (n = 3/group, Kruskal–Wallis, Dunn’s post-test *p = 0.0332 versus MCs; n = 3/group, Kruskal–Wallis, Dunn’s post-test) and in (right) total retinal explants cultured 18 h with or without IL-1β (50 ng/ml) (n = 5/group). b RT-qPCR of Eaat2 mRNA normalized with the mean of 3 HKG mRNAs in retinal cell fractions isolated from (left) total retina and in (right) total retinal explants cultured 18 h with or without IL-1β (50 ng/ml) (n = 5/group, Mann–Whitney test; n = 5/group, Mann–Whitney test, *p = 0.0183). c RT-qPCR of Eaat2 mRNA normalized with the mean of 3 HKG mRNAs in (left) Müller cell fraction (n = 6/group, Mann–Whitney test, *p = 0.0360) and (right) PRs isolated from retinal explants cultured with or without IL-1β (50 ng/ml) for 18 h (n = 6/group). d RT-qPCR of xCT mRNA normalized with the mean of 3 HKG mRNAs in retinal cell fractions isolated from total retina (n = 4/group, Kruskal–Wallis, Dunn’s post-test *p = 0.0128 versus MCs). e (left) RT-qPCR of xCT mRNA normalized with the mean of 3 HKG mRNAs in retinal explants cultured with or without IL-1β (50 ng/ml) for 18 h (n = 6/group, Mann–Whitney test, *p = 0.0089). (right) Western blot analysis of XCT in retinal explants cultured with or without IL-1β (50 ng/ml) for 18 h (n = 5/group, Mann–Whitney test, *p = 0.0159). f RT-qPCR of xCT mRNA normalized with the mean of 3 HKG mRNAs in (left) Müller cell (n = 6/group, Mann–Whitney test, **p = 0.0303) and (right) PR fractions (n = 6/group) isolated from retinal explants cultured 18 h with or without IL-1β (50 ng/ml). g RT-qPCR of Gs mRNA normalized with the mean of 3 HKG mRNAs in Müller cell fraction isolated from retinal explants cultured with or without IL-1β (50 ng/ml) for 18 h (n = 6/group, Mann–Whitney test, *p = 0.0152). h Intracellular glutamate molar quantity (pmol/cell) measured in the lysate of the Müller cell fraction isolated from retinal explants cultured 18 h with or without IL-1β (50 ng/ml) (n = 5/group, Mann–Whitney test, *p = 0.0152)

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