Fig. 4

Microglial cells do not participate directly to glutamate homeostasis disruption induced by IL-1β. a Orthogonal projection of confocal Z stack images of the outer nuclear layer of IBA1 (green)- and Hoechst nuclear dye (blue)-stained mouse retinal explants after 18 h of culture with or without IL-1β (50 ng/ml). b Number of IBA1-postive cells per mm² in the outer nuclear layer of mouse retinal explants after 18 h of culture with or without IL-1β (50 ng/ml) (n = 4 per group). c Quantification of the cell perimeter and the soma area of microglia from retinal explants cultured 18 h with or without IL-1β (50 ng/ml) (t test, *p = 0.0133 and *p < 0.0001 respectively, n = 79 and 52 for CTL and Il-1β respectively). d RT-qPCR of xCT mRNA normalized with the mean of 3 HKG mRNAs in isolated microglial cells cultured with or without IL-1β (50 ng/ml) (n = 4/group, Mann–Whitney). e Glutamate concentration (μM) measured in the supernatant of isolated microglial cells cultured with or without IL-1β (50 ng/ml) (n = 4/group, Mann–Whitney)