Fig. 6

uPAR-specific antibody neutralizes the effects of Plg on cytokine expression by N-astrocytes. (a–c) N-astrocytes were treated with Plg (0.2 μM), Plg plus tPA (12 nM), or vehicle in presence and absence of uPAR-specific antibody (1 μg/mL) or an equivalent concentration of non-specific IgG for 6 h. Expression of TNFα, IL-1β, and CCL2 was determined by RT-qPCR (mean ± SEM; n = 3; ***p < 0.001; one-way ANOVA with Tukey’s post hoc test. Statistical analysis is relative to the vehicle control). d N-astrocytes were treated with Plg (0.2 μM), Plg plus tPA (12 nM) or vehicle in presence and absence of uPAR-specific antibody (1 μg/mL) or an equivalent concentration of non-specific IgG. Immunoblot analysis was performed to detect phospho-IκBα, IκBα and β-actin