Fig. 7

The uPA ATF blocks cytokine expression by N-astrocytes in response to Plg. a–c N-astrocytes were treated for 6 h with Plg (0.2 μM) and the indicated concentrations of uPA ATF. Expression of TNFα, IL-1β, and CCL2 was determined by RT-qPCR (mean ± SEM; n = 3; ***p < 0.001; one-way ANOVA with Tukey’s post hoc test. Statistical analysis is relative to the vehicle control). d N-astrocytes were treated for 1 h with Plg (0.2 μM) and the indicated concentrations of uPA ATF. Immunoblot analysis was performed to detect phospho-IκBα, IκBα, and β-actin