Fig. 2

Expression and knockdown of α-Syn and SNCA in the spinal cord following SCI. A Representative Western blot results of α-Syn and p-α-Syn levels in the SCI group compared with the sham group at 1, 7, 14, and 28 days after SCI. B, C Quantification of the Western blot data in panel A, n = 5. D Relative expression levels of SNCA in the sham group and the SCI group over time, as detected by qRT-PCR, n = 5. E IHC was used to detect the expression of α-Syn in the GM and WM of the spinal cord on the 28th day after SCI. Scale bar = 100 μm. F, G Semi-quantitative analysis of the mean IOD values from IHC in panel E, n = 5. H Relative expression levels of SNCA in the three groups, as detected by qRT-PCR at 28 dpi, n = 5. I Distribution of lentivirus (LV; GFP+) expression in the spinal cord after SCI. Scale bar = 3 mm. Green fluorescence indicates a tissue transfected with LV. All data are presented as the mean ± SD. Comparisons between two groups were performed using Student’s t test or the Mann-Whitney test, as appropriate. One-way ANOVA and Tukey’s multiple comparisons test were used to analyse differences among groups