Fig. 9

Reductions in α-Syn can reduce microglial/astrocyte activation in injured spinal cord tissue. A Representative images showing GFAP+/Iba1+ double staining after SCI by IF (a, e, and i show microglia (Iba1); b, f, and j show microglia (Iba1); c, j, and k show nuclei (DAPI); and d, h, and i show the 3D mode). Scale bar = 50 μm. B Quantitative analysis of the mean fluorescence intensity of GFAP in panel A. C Quantitative analysis of the mean fluorescence intensity of Iba1 in panel A. All data are presented as the mean ± SD, n = 5. One-way ANOVA and Tukey’s multiple comparisons test were used to analyse differences among groups