Fig. 2

BAP31-deficient BV2 cells produce more inflammatory cytokines. Scramble and shBAP31 were treated with LPS (100 ng/ml) for 4 h. The mRNA levels of the cytokines IL-1β (a), TNFα (b), and COX2 (c) were analyzed with RT-PCR. Scramble and shBAP31 were treated with LPS (100 ng/ml) for 24 h. The protein levels of the cytokines IL-1β (d, g), TNFα (e, h), and COX2 (f, i) in cells were analyzed with Western bloting; the secreted protein levels of the cytokines IL-1β (j) and TNFα (k) in supernatant were analyzed with ELISA kits. All the data are indicated as Mean ± SEM of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001 versus control group