Fig. 3

BAP31 deficiency facilitates p65 and c-Jun nuclear accumulation and transcriptional activity. Scramble and shBAP31 BV2 cells were treated with LPS for 30 min; the cytosolic (a) and nuclear (d) fractions of p65 and c-Jun were analyzed by Western blotting with antibodies to c-Jun, p65, histone, and β-actin. Immunoblots for c-Jun (b) and p65 (c) in cytosolic fractions were quantified and normalized to β-actin protein. Immunoblots for c-Jun (e) and p65 (f) in nuclear fractions were quantified and normalized to histone protein. g, h Scramble and shBAP31 Raw264.7 cells were cotransfected with NF-κB luciferase reporter plasmid or AP-1 luciferase reporter plasmid and pRL-SV40-C plasmid for 48 h, and p65 and c-Jun reporter activities were analyzed following treatment with LPS for 24 h. All the data are indicated as Mean ± SEM of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001 versus control group