Fig. 4

Effect of BAP31 deficiency on the protein levels of IRAK1 and MyD88 in BV2 cells. a, b Scramble and shBAP31 BV2 cells were treated with LPS for 0, 5, and 15 min. Representative Western blots showing the expression levels of IRAK1 and MyD88. c, d Representative Western blots showing the effect of BAP31 on the protein level of IRAK1 in primary microglia and HEK293T cells. e Quantitative RT-PCR measured the relative mRNA expression of IRAK1 in primary WT and KO microglial cells. f Quantitative RT-PCR measured the relative mRNA expression of IRAK1 in scramble and shBAP31 BV2 cells. All the data are indicated as Mean ± SEM of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001 versus control group