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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: BAP31 regulates IRAK1-dependent neuroinflammation in microglia

Fig. 6

Effect of BAP31 deficiency on the nuclear factor-κB and mitogen-activated protein kinase (MAPK) pathways in BV2 cells. Scramble and shBAP31 BV2 cells were treated with LPS for 0 min, 5 min, 15 min, 30 min, 1 h, 2 h. a Representative Western blots showing the expression levels of total and phospho-p65, IκBα, phospho-IKKα/β, and total and phospho-TAK1. The intensity of the phospho-p65 (b) and phospho-TAK1 (e) protein bands were quantified and shown as the ratio of phosphorylated protein/total protein to control after normalization to β-actin. The intensity of the IκBα (c) and phospho-IKKα/β (d) proteins bands were quantified and shown as the ratio to control after normalization to β-actin. f Representative Western blots showing expression levels of total and phospho-JNK, total and phospho-p38. The intensity of the phospho-JNK (g) and phospho-p38 (h) protein bands were quantified and shown as the ratio of phosphorylated protein/total protein to control after normalization to β-actin. All the data are indicated as Mean ± SEM of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001 versus control group

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